Detection of other special sample please contact our technical support. Whether youre looking for sandwich, direct, indirect, competitive, inhibition, bridging, capture or another elisa protocol. The enzyme linked immunosorbent assay elisa is a powerful method for detecting and quantifying a specific protein in a complex mixture. Prostaglandin e2 pge2 is formed in a variety of cells from prostaglandin h2, which is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. Like most eicosanoids, it does not exist preformed in any cellular reservoir.
Screen inhibitors of cox ii activity by measuring the levels of downstream pge 2. Csbe17168ha for the quantitative determination of endogenic hamster prostaglandin e2 pge2 concentrations in serum, plasma and. The specificity of the pge2 elisa was investigated using authentic pge2 and fatty acids which, based on their structure, might be anticipated to compete with pge2 for binding to antibodies against pge2. Prostaglandin e2 pge2 is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Endogenous pge2 promotes the induction of human th17. Duoset elisa kits can be used to develop a sandwich immunoassay and are an economical alternative to buying separate antibodies and protein standards. Parameter pge2 assay catalog number kge004 for the quantitative determination of prostaglandin e2 pge2 in cell culture supernates, serum, plasma, and urine. Pge2 induced in and released by dying cells functions as an.
Overview abcam s prostaglandin e2 pge2 in vitro competitive elisa enzymelinked immunosorbent assay kit is designed for the accurate quantitative measurement of prostaglandin e2 in serum, saliva, urine and tissue culture media and other biological fluids. Synonyms prostaglandin e2,dinoprostone, search name canine prostaglandin e2 elisa kit, prostaglandin e2 elisa kit, dinoprostone elisa kit intended use. Ellsworth rd ann arbor, mi usa prostaglandin e 2 elisa kit monoclonal item no. Porcine prostaglandin e2 pge2 elisa kit this product is suitable for in vitro quantitative detection of porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant pge2. This eia kit allows for the widest variations in sample size, sensitivity and assay timing of any pge 2 kit. Standards, test samples, and biotinconjugated reagent are added to the wells and incubated. A competitive inhibition reaction takes place between the the precoated pge2 and the pge2.
Pge2 was measured using a competitive elisa kit from cayman chemical co. Biovisions pge2 elisa kit is a competitive elisa assay for the quantitative measurement of pge2 in serum, plasma and cell culture supernatants. Pge2 elisa kit colorimetric ka0326 these standard curves are provided for demonstration only. C the excess reagents are washed away and substrate is added. Prostaglandin e2 elisa kit okeh02544 quantitative competitive. The microtiter plate provided in this kit has been precoated with an antibody specific to prostaglandin e2, during the. Pge2 prostaglandin e2 elisa kit lsf32354 is a 96well enzymelinked immunosorbent assay for the quantitative detection of mouse pge2 prostaglandin e2 in plasma, serum, tissue homogenates. Kge004b contains sufficient materials to run an elisa on one 96 well plate. Split samples into two equal parts and place each sample into clean test tubes. Pge2 prostaglandin e2 elisa kit lsf27108 is a 96well enzymelinked immunosorbent assay for the quantitative detection of human pge2 prostaglandin e2 in plasma, serum. Pge2 is the main product of two cyclooxygenases, cox1 and cox2, of which cox1 is constitutively expressed and cox2 is induced by various stimuli such as cytokines and various pamps 12. Rebag any unused strips and frames, and store at 2 to 8c for future use. General information 3 table of contents general information 3 materials supplied. As todays scientists demand high quality consistent data for high impact journals, elisa genie have developed a range of sensitive, fast and reliable elisa kit assays to meet and exceed those demands.
The pge 2 eia kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Assay protocol 20 preparation of assayspecific reagents 22 plate set up. Pge2 promotes breast cancerassociated lymphangiogenesis by. It is a competitive eia assay which can detect pge2 prostaglandin e2 down to 31. Human pge2 prostaglandin e2 quant elisa kit sandwich. This elisa kit is used for in vitro quantitative determination of pge2. This package insert must be read in its entirety before. Dilute the antigen to 12 ugml in coating solution 2. The human il2 elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of human il2 in serum, plasma, cell culture. Porcine prostaglandin e2 pge2 elisa kit this product is suitable for in vitro quantitative detection of porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant pge2 concentration. The elisa genie rat prostaglandin e2 pge2 elisa kits are designed for the competitive measurement of analytes in a wide variety of samples. Ehpge2 product description a competitive immunoassay for the quantitative determination of prostaglandin e. This can be stored at room temperature until the kit expiration date, or.
Caymans pge2 elisa kit monoclonal is a competitive assay that can be used for quantification of pge2 in urine, plasma, tissue culture supernatants, and other. As explained on page 11 of the protocol booklet, in order to help preserve the pge2, we. Mouse pge2 prostaglandin e2 elisa kit competitive eia. It is a competitive eia assay which can detect pge2. Any unused conjugate should be aliquoted and refrozen at or below 20 c. Depending on the protein of interest, antibodies, buffers, or substrates being used, this general protocol. A competitive inhibition reaction takes place between the the precoated pge2 and the pge2 in the sample with the biotinlabelled antibody. Screen inhibitors of cox ii activity by measuring the levels of. This elisa kit used for quantitative determination of pge2 in serum, plasma and other biological. Pge2 multiformat elisa kit arbor assays arbor assays. Prostaglandin e elisa kit monoclonal cayman chemical.
This assay is designed to detect and quantify the level of human prostaglandin e2 in biological samples. Rat prostaglandin e2 pge2 elisa kit this product is suitable for in vitro quantitative detection of rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant pge2 concentration. The kit uses a monoclonal antibody to pge2 to bind, in a competitive manner, the pge2 in the sample or an alkaline phosphatase molecule which has pge2 covalently attached to it. Oxidation of arachidonic acid by prostaglandin synthases cox1 and cox2 produces prostaglandin h2 pgh2, which is further metabolized by pge synthases into its major product, pge2. In support of cox2 upregulation, these cells also exhibited an increase in endogenous pge2 production as shown with elisa in the cell lysate fig. Prostaglandin e 2 pge 2 is a primary product of arachidonic acid metabolism in many cells. Overview abcam s prostaglandin e2 pge2 in vitro competitive elisa enzymelinked immunosorbent assay kit is designed for the accurate. Prostaglandin e2 elisa kit okeh02544 assay methodology. Canine prostaglandin e2, pge2 elisa kit 96 tests operating instructions for research use only. Tissuecell lysate have not been internaly validated so we do not have specific protocols. Elisa enzymelinked immunosorbent assay is a platebased assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones.
It is a sandwich assay which can detect pge2 prostaglandin e2 down to 2. Reconstitute the 100 dtn pge2 tracer with 6 ml eia buffer. The sandwich elisa protocol provided here is representative of most readytouse elisa kits for measurement of cytokines, chemokines, growth factors, and other extracellular targets. This protocol works well for most samples, but may need optimization for sufficient purification of pge2 from all samples. The mouse il2 elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of mouse il2 in serum, plasma and cell. Elisa procedure determine the number of 8well strips required for the assay. Tissuecell lysate have not been internaly validated so we do not have specific protocols for them however a number of users have had successful results using these sample types. Wash buffer prepare the wash buffer by diluting 5 ml of the supplied concentrate with 95 ml of deionized water. View all protocols, troubleshooting, illustrated assays and webinars. The micro elisa plate provided in this kit has been precoated with pge2. Pge2 elisa kit standard curve pge2 elisa kit lot to lot performance. Please refer to references 1518 for details of extraction protocols. Protocol summary prepare all reagents, samples, and standards as instructed add standards and samples to appropriate wells.
Hamster prostaglandin e2 pge2 elisa kit catalog number. Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation. Below are two protocols for measuring pge2 in plasma or serum using starting volumes of 1. This competitive elisa kit is for determination of pge2 prostaglandin e2 levels in biological samples. Elisa kit for prostaglandin e2 pge2, competition elisa. Prostaglandin e2 pge2 elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Cea538ge, elisa kit for prostaglandin e2 pge2, panspecies general, competition elisa, pg e2, designed by cloudclone corp. Pge2 elisa for the quantitative determination of prostaglandin e2 in cell culture, saliva, serum, urine and whole blood. This pge2 elisa kit is a competitive assay that can be used for quantification of pge2 and has a range from 7. A standard curve should be generated for each set of samples assayed. Elisa basics guide 5 elisa technology elisa technology elisa formats the first step in an elisa experiment is the immobilization of the antigen in a sample to the wall of the wells of a microtiter plate.
The density of color is proportional to the amount of pge2. The kit uses a monoclonal antibody to pge2 to bind, in a competitive manner, the pge2 in the sample, standard, or an alkaline phosphatase molecule which has pge2 covalently attached to it. The assay is based on the competition between pge 2 in the standard or sample and alkaline phosphatase. In vivo, pge 2 is rapidly converted to an inactive metabolite,14dihydro15keto. Use within 4 weeks of reconstitution and store 4 c. Prostaglandin e2 pge2 elisa kit e4637 biovision, inc. This elisa kit is used for in vitro quantitative determination of pge2 in samples. Elisa technical guide and protocols thermo fisher scientific. Prostaglandin e2 pge2 is produced in multiple cell types from pgh2, a primary product of the arachidonic acid metabolism, via the prostaglandin synthase. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. Pge2 conjugate allow the conjugate to warm to room temperature. The density of color is proportional to the amount of pge2 captured from the samples.
Human pge2 prostaglandin e2 quant elisa kit sandwich lsbio. Catalog number kge004b catalog number skge004b catalog number pkge004b for the quantitative determination of prostaglandin e 2 pge 2 in cell culture supernates, serum. Add 100 ul of diluted antigen to appropriate wells. Supernatants from the mlr were collected after 7 days of culture and evaluated for il17 production by elisa. As todays scientists demand high quality consistent data for high impact journals, elisa genie have developed a range of sensitive, fast and reliable elisa. Elisa kit is designed to quantitatively measure pge 2 present in serum, plasma, urine, saliva, cells, tissue, and tissue culture media samples. Perform the elisa for pge2 according to the instructions of the manufacturer.
Human il2 elisa kit for serum, plasma, cell culture. Association between the levels of prostaglandin e2 in. Faqs for pge2 elisa kit nbp229336 0 there are no specific faqs related to this product. Pge2 has a number of biological actions, including vasodilation, both anti and proinflammatory action, modulation of sleepwake cycles, and facilitation of human immunodeficiency. The elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. The prostaglandin e2 kit is designed for the rapid detection of pge2 in cell supernatant and whole cells. The assay will recognize both natural and recombinant human. Allow kit components to come to room temperature for at least 30 minutes before use. Rat prostaglandin e2 pge2 elisa kit this product is suitable for in vitro quantitative detection of rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant pge2.
I would appreciate any suggestion on elisa kits to measure pge2 from mouse tissue and if you can comment on sample preparation or you have a protocol that worked for you. The elisa is based on the competitive binding enzyme immunoassay technique. Please read the complete kit insert before performing this assay. Originally described by engvall and perlmann 1971, the method. Mouse il2 elisa kit for serum, plasma and cell culture. Pge2 promotes breast cancerassociated lymphangiogenesis. Pge2 elisa kit enzo life sciences adi900001 product. Apr 05, 2016 pge2 is an eicosanoid lipid mediator that can exert various effects including cell proliferation, stem cell expansion, and immunosuppression. Originally described by engvall and perlmann 1971, the method enables analysis of protein samples immobilized in microplate wells using specific antibodies. Pge2 displays several biological functions, such as vasodilatation and smooth muscle relaxation, and is involved in pro and antiinflammation pathways. It is recommended that the urine sample be diluted 4fold with 1x sample dilution buffer and 100 ul of sample added directly to the elisa plate well. Depending on the protein of interest, antibodies, buffers, or substrates being used, this general protocol may need to be optimized. Add prepared labeled apconjugate to appropriate wells. Prostaglandin e 2 assay parameter tm this package insert must be read in its entirety before using this product.
Jan 05, 2017 in support of cox2 upregulation, these cells also exhibited an increase in endogenous pge2 production as shown with elisa in the cell lysate fig. Incubate 2 hours at room temperature or 4 c overnight. The elisa genie mouse prostaglandin e2 pge2 elisa kits are designed for the competitive measurement of analytes in a wide variety of samples. During the reaction, pge2 in the sample or standard competes with a fixed amount of pge2 on the solid phase supporter for sites on the biotinylated detection ab specific to pge2. This elisa kit uses the competitive elisa principle. A competitive immunoassay for the quantitative determination of prostaglandin e2 in biological. Prostaglandin e2 monoclonal ab solution preparation. The detectx prostaglandin e2 pge2 multiformat elisa kit is designed to quantitatively measure pge2 present in serum, plasma, urine, tissues, saliva and culture media samples. Since c3l5cm was known to contain pge2 in addition to other molecule such as vegfc and d, we could not distinguish amongst these molecules which exerted the effects.